|
Isolation of protein from lentil |
|---|---|
| รหัสดีโอไอ | |
| Title | Isolation of protein from lentil |
| Creator | Zar Zar Oo Thwe Linn Ko Soe Soe Than |
| Contributor | Industrial Chemistry Department Yadanabon University Mandalay Myanmar |
| Publisher | Mahasarakham University |
| Publication Year | 2560 |
| Keyword | Lentil flour Defatted flour Soxhlet extraction, Isoelectric precipitation Lentil protein isolate |
| Abstract | The main purpose of this research work was to combat the problem of malnutrition and to isolate the most refined form of protein from lentil bean for food processing. In this research work, lentils (Lens culinaris L.) was collected from Monywa Township, Sagaing Region and nutritional characteristics such as moisture content, ash content, fat content, carbohydrate content, protein content, fiber content and energy value were determined. The fat of raw bean flour was removed by bulk soaking in ethanol and also by soxhlet extraction using ethanol as solvent before isolating the protein. In addition, the fiber and starch from defatted lentil flour was removed by alkaline extraction and acid precipitation method to isolate the protein (isoelectric precipitation). Protein solubility, water and oil absorption capacity, emulsifying capacity and stability, foaming capacity and stability of lentil protein isolate have been determined. The solubility curve corresponding to the lentil protein isolate indicated the minimum solubility at pH 4 (protein solubility of 20 %) and maximum solubility at pH 12 (protein solubility of 85 %) respectively. The lentil protein isolate had water absorption capacity of 1.82 ml H2O/g. protein and oil absorption capacity of 1.95 ml oil/g. protein. It was found that emulsion stability of isolated lentil protein was 41%with foaming capacity was 22.67 %., The foam stability was preserved up to 150 min. Isolated lentil protein improved texture appearance and taste than the lentil flour and thus it can better be used as nutrition and functional ingredients in many food products |
