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Heparan sulfate is a potential receptor for VP37 of white spot syndrome virus |
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| รหัสดีโอไอ | |
| Title | Heparan sulfate is a potential receptor for VP37 of white spot syndrome virus |
| Creator | Apisit Lueangyangyuen Anchulee Saleeart Triwit Rattanarogpong Ha Thanh Dong, Saengchan Senapin Kanokpan Wongpresert Pongsak Khunrae |
| Contributor | Department of Microbiology Faculty of Science King Mongkut's University of Technology Thonburi KMUTT Bangkok 10140 Thailand |
| Publisher | Mahasarakham University |
| Publication Year | 2560 |
| Keyword | WSSV VP37 Heparan-like glycosaminoglycans |
| Abstract | VP37 of White Spot Syndrome Virus (WSSV) is believed to be an viral envelope protein responsible for the attachement of WSSV to shrimp heamocytes. However, the shrimp molecule that is targeted by VP37 is still unknown. Here in this study, we demonstrated through ELISA assay that the binding of VP37 to shrimp heamocytes could be prevented by soluble sulfated galactan (SG) extracted from red seaweed (Gracilaria fisheri). This observations allowed us to hypothesize that VP37 might be able to recognize SG-like molecules presented on the surface of shrimp heamocytes. Since there is a report on the presence of heparan sulfate (HS) in shrimp tissues, we then performed Surface Plasmon Resenance (SPR) analysis to investigate the binding of VP37 to HS. Our SPR analysis showed that VP37 strongly bound to HS with the binding affinity of 1 ตM. Interestingly, it was also shown through SPR assay that the binding of VP37 to HS could be blocked by SG. This prompted us to propose that the attachment of WSSV on shrimp tissue is mediated by the binding fo VP37 to HS presented on the surface of shrimp tissue and that prevention of this interaction by SG can help reduce infectivity of WSSV in shrimp as previously seen in other previous studies. |
