|
Effect of cane sugar on glucosidase activity in the fermentation of plu- Kao juice using lacticacid bacteria starters |
|---|---|
| รหัสดีโอไอ | |
| Title | Effect of cane sugar on glucosidase activity in the fermentation of plu- Kao juice using lacticacid bacteria starters |
| Creator | Papatchaya Kontongdee |
| Contributor | - |
| Publisher | Mahasarakham University |
| Publication Year | 2559 |
| Keyword | Plu-kaojuice ,Cane sugar, Flavonoids aglycone, Lactic acid bacteria, ?-glucosidase, |
| Abstract | Plu-Kao, a traditional medicinal plant in East Asia and the West,is currently being processed by fermenting plu-kao juice with cane sugar. Lactic acid bacteria were recovered and isolated from fermented Plu-kao juice.Of these it was noted that 14 isolates were found to possess ?-glucosidase activity on MRS+3% esculin.?-glucosidase is an enzyme that catalyzes the cleavage of the ?-1-4 glycosidic bond of flavonoid glycoside resulting in (i) flavonoid aglycone, an active form of flavonoid glycoside that serves as a more potent antioxidant, and (ii) a free moiety of ?D-glucose.The 14 LAB isolates were analyzed for ?-glucosidase activity by cultivating each isolate on modified MRS+cane sugar at 0.5, 1, 2, 3, 7, and 7.5%w/v.The results showed that strains LH 26, LH 59, LH 56 and LH 55 possessed the highest ?-glucosidase activities at 17.82, 18.79, 21.81 and 19.92 U/ml when varying the concentrations as 0.5, 1, 7 and 7.5 % w/v respectively, while strain LH 49 were found to possess highest enzyme activity as 27.66 and 26.77 U/ml when cultivating on Modified MRS + cane sugar as 2 and 3 % w/v respectively when determined at 37 oC.The enzyme activities of these 14 positive strains grown on MRS at each sugar concentration given above were in the ranges 8.87 17.82, 5.40 18.39, 13.15 - 27.66, 4.52- 27.02, 10.44- 21.81and 5.65 19.92U/ ml respectively.In conclusion, the concentration of cane sugar has affected the enzyme activities among the 14 strains, thus optimal concentration of cane sugar can help to enhance this enzyme activity. |
