|
Biotransformation of 3-Hydroxypropionaldehyde from Glycerol by Isolated Lactic Acid Bacteria |
|---|---|
| รหัสดีโอไอ | |
| Title | Biotransformation of 3-Hydroxypropionaldehyde from Glycerol by Isolated Lactic Acid Bacteria |
| Creator | Preeyawan Phaliwong |
| Contributor | - |
| Publisher | Mahasarakham University |
| Publication Year | 2559 |
| Keyword | Isolated lactic acid bacteria, biotransformation, 3-Hydroxypropionaldehyde (3-HPA) |
| Abstract | 3-Hydroxypropionaldehyde (3-HPA) is a small chemical molecule which can be used as an antimicrobial substance and intermediate for the synthesis of acrylic acid. 3-HPA can be generated through the biotransformation of glycerol by glycerol dehydratase. Somestrains of lactic acid bacteria have glycerol dehydratase for 3-HPA production. Therefore, the biotransformation of glycerol to 3-HPA by isolated lactic acid bacteria from piglet intestine was investigated. This study used Lactobacillus sp. LAB100 and Lactobacillus sp. LAB001 as cells for 3-HPA production by comparison with the reference strain, Lactobacillus reuteriDSM20016. A 30% glycerol solution (10 ml) was transformed to 3-HPA at 37?C for 24 h by cell producers (stationary phase). The 3-HPA concentration and antimicrobial activity were then determined. Results showed that Lactobacillus sp. LAB100, Lactobacillus sp. LAB001, and L. reuteri DSM20016 gave 3-HPA concentrations of 64.5, 58.2, and 73.7 g/l, respectively. The two isolates (LAB100 and LAB001) displayed antimicrobial activity against Salmonella typhimurium TISTR292, with similar activity toward the reference strain L. reuteri DSM 20016 (1600 AU/ml). |
