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Effect of Putrescine and Sucrose on Vase Life of Dendrobium cv. 5N |
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| รหัสดีโอไอ | |
| Title | Effect of Putrescine and Sucrose on Vase Life of Dendrobium cv. 5N |
| Creator | Israporn Unsanan |
| Contributor | Khon Kaen University |
| Publisher | Mahasarakham University |
| Publication Year | 2559 |
| Keyword | Dendrobium, putrescine, vase life, holding solution |
| Abstract | The effect of different concentrations of putrescine and sucrose on the vase life of Dendrobium cultivar 5N was investigated. Dendrobium inflorescences were immersed in putrescine (0.25, 0.50, 1, and 2 mM) and sucrose solutions (1, 2, 4, and 6%) for 4 h at 25?2?C, then transferred into deionized water and kept in the culture room at 25?2?C. Putrescine at 2 mM significantly increased the vase life of Dendrobium cultivar 5N flowers (24.37 days) compared to the control (20.87 days). A holding solution containing putrescine at 2 mM and sucrose at 1% also prolonged the vase life of the orchid flowers (20.28 days). Vase life of Dendrobium flowers treated with 2 mM putrescine alone, or a mixture of 2 mM putrescine and 1% sucrose was comparable to flowers treated with silver thiosulfate (STS) and a commercial holding solution. The effect of putrescine on the expression of the ACS and ACO genes was determined by reverse transcription polymerase chain reaction (RT-PCR) using primers synthesized on the ACS and ACO genes from the orchid flowers, and RNA isolated from putrescine treated flowers as the template. Results revealed that putrescine suppressed expression of the ACS and ACO genes similar to STS treatment, and suggested that putrescine and sucrose have high potential as a holding solution for prolonging the vase life of Dendrobium cultivar 5N. |
