|
Antioxidant Activity of Casein Hydrolysate |
|---|---|
| รหัสดีโอไอ | |
| Title | Antioxidant Activity of Casein Hydrolysate |
| Creator | Kritsada Pumpeth |
| Contributor | - |
| Publisher | Mahasarakham University |
| Publication Year | 2559 |
| Keyword | Casein, casein hydrolysate, bioactive peptide, antioxidant, degree of hydrolysis Introduction |
| Abstract | Casein hydrolysate, obtained by the hydrolysis of casein under suitable conditions, contains bioactive casein peptides which have positive effects on health. This study prepared high antioxidant activity casein hydrolysate using alcalase and trypsin. Antioxidant activity of the casein hydrolysate samples was evaluated through DPPH and ABTS radical scavenging activity. The effect of hydrolysis time on the degree of hydrolysis was also investigated. Free amino acid levels increased with hydrolysis time with 8.18 g/100g at the beginning and 60.92 g/100g of casein hydrolysate at 180 min.Casein hydrolysate obtained from trypsin hydrolysis at 180 min indicated highest antioxidant activity using DPPH radical scavenging activity, expressed as ascorbic acid (VC) equivalent and Trolox equivalent with the activity of 33.7 mg VC equivalent/ml and 38.8 mg Trolox equivalent/ml, respectively. Casein hydrolysate obtained from alcalase hydrolysis indicated lower activity than from trypsin at the same hydrolysis time. Antioxidant activity using ABTS scavenging assay indicated that casein hydrolysate obtained from trypsin hydrolysis at 180 min had the strongest antioxidant activity at 38.9 mg VC equivalent/ml and 38.8 mg Trolox equivalent/ml. Results demonstrated that hydrolysis time influenced antioxidant activity, with longer hydrolysis time producing significantly higher antioxidant activity. |
