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Preparation of bioethanol from lignocellulosic biomass (sugarcane bagasse) by acid hydrolysis |
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| รหัสดีโอไอ | |
| Title | Preparation of bioethanol from lignocellulosic biomass (sugarcane bagasse) by acid hydrolysis |
| Creator | Thu Ya Tun and Khin Mar Hlaing |
| Contributor | Industrial Chemistry Department, Yadanabon University, Mandalay, Myanmar |
| Publisher | Mahasarakham University |
| Publication Year | 2558 |
| Keyword | Bioethanol, Sugarcane Bagasse, Acid Hydrolysis, Fermentation, Saccharomyces Cerevisiae. |
| Abstract | Bioethanol has been considered for use as alternative fuel instead of increasingly expensive petroleum supplies. In this research, bioethanol was prepared from lignocellulosic biomass such as sugarcane bagasse. Cellulose hydrolysis was conducted by using acids such as hydrochloric acid and sulphuric acid with various acid concentrations, volume of acids and hydrolysis time to obtain the maximum yield of reducing sugar. In these experiments, the suitable conditions to obtain maximum yield of reducing sugar were hydrolyse 30g (6% w/v) sugar cane bagasse with 3% H2SO4 and 4% HCl for 4h and 5h, respectively. After acid hydrolysis, fermentation was carried out by using Saccharomyces cerevisiae under anaerobic conditions. In this experiment, amount of yeast and fermentation time were also varied to find the most suitable condition of alcohol strength. The most suitable amount of yeast for sulphuric acid and hydrochloric acid were 2.5g/l and 3.5g/l but the fermentation time for 5 days was the same for both hydrolysis. And then the fermentation broth was separated by distillation method to obtain bioethanol. In spite of varying the parameters of distillation time were conducted to optimize alcohol strength and maximum yield of bioethanol, the most suitable time was found to be 3 hours for both acid hydrolysis. |