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The neuro-protective effects of curcumin against I/R injury in transient MCAO rat model |
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| รหัสดีโอไอ | |
| Title | The neuro-protective effects of curcumin against I/R injury in transient MCAO rat model |
| Creator | Wei Li |
| Contributor | Suthiluk Patumraj, Nijasri Charnnarong |
| Publisher | Chulalongkorn University |
| Publication Year | 2558 |
| Keyword | Brain -- Blood-vessels, Cerebrovascular disease, สมอง -- หลอดเลือด, โรคหลอดเลือดสมอง |
| Abstract | Oxidation, inflammation, and apoptosis are three critical factors for the pathogenic mechanism of cerebral ischemia/reperfusion (I/R) damage. Curcumin has been elucidated to exhibit substantial biological properties via anti-oxidation, anti-inflammation and anti-apoptosis effects, however, its molecular mechanism against cerebral I/R injury remains unclear. To investigate the effects of curcumin on cerebral I/R injury associated with water content, infarction volume, blood-brain barrier (BBB) disruption and the expression of ICAM-1, MMP-9, NF-kappa-B, caspases-3, and Nrf2. The middle cerebral artery occlusion (MCAO, 1- hour occlusion and 24-hour reperfusion) was performed in male Wistar rats as representing cerebral I/R injury model. In MCAO+CUR group, rats were received curcumin administration (300 mg/kg BW, ip.) at 30-min after occlusion. The same operated procedures were performed in SHAM rats without MCAO occlusion. At 24-hour post-operation, all of these parameters including neurological deficit scores, BBB disruption, water content, and infarction volume were determined. Brain tissue ICAM-1, MMP-9, NF-kappa-B, caspases-3, and Nrf2 were assayed by immunohistochemistry. Compared with SHAM group, the BBB disruption, neurological deficit scores, brain water content and infarction volume were severely demonstrated in MCAO group. ICAM-1, MMP-9, NF-kappa-B and caspases-3 were enhanced in MCAO group. However, in MCAO+CUR group, the upregulated Nrf2, an anti-oxidation related protein, collaborating with the decline of other biomarkers were significantly observed. The protective effects of curcumin against cerebral I/R injury were attributed to its anti-oxidation, anti-inflammation and anti-apoptosis which were involved in the up-regulation of Nrf2 and the down-regulation of ICAM-1, MMP-9, NF-kappa-B and caspases-3. |
| URL Website | cuir.car.chula.ac.th |
