ASTAXANTHIN INDUCTION OF HAEMATOCOCCUS PLUVIALIS IN FLAT PANEL AIRLIFT PHOTOBIOREACTORS
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Title ASTAXANTHIN INDUCTION OF HAEMATOCOCCUS PLUVIALIS IN FLAT PANEL AIRLIFT PHOTOBIOREACTORS
Creator Woradej Poonkum
Contributor Prasert pavasant, Sorawit Powtongsook
Publisher Chulalongkorn University
Publication Year 2556
Keyword Biological assay, Antioxidants, Microalgae, การสอบปริมาณโดยชีววิธี, แอนติออกซิแดนท์, สาหร่ายขนาดเล็ก, ปริญญาดุษฎีบัณฑิต
Abstract This work studied astaxanthin induction from H.pluvialis (NIES-144) using flat panel airlift photobioreactors (FP-APBRs) under indoor and outdoor conditions. Preliminary experiments in 1.5 L bubble column photobioreactors (BC-PBR) revealed that sterilized clean water with 3% CO2 aeration (1.47 cm3 s-1 CO2 loading), 35,000 LUX and 330C could best encourage astaxanthin accumulation at 18.21 g m-3 (3.63% by weight). Operating 17 L FP-APBR with these bubble column parameters under the downcomer to riser cross sectional area ratio (Ad/Ar) of 0.4 and superficial gas velocity of 0.4 cm s-1 could further enhance astaxanthin to 26.63 g m-3 (5.34% by weight). For the operation of the one-step process system, reducing nitrate and phosphate at 4 and 6-fold of that in the original F1 medium could best encourage astaxanthin accumulation at 28 g m-3 (5.24% by weight). However, similar operation under outdoor condition exhibited slightly poorer performance due to the light inhibition effect. The best outdoor performance was obtained with the FP-APBR covered with 1-layer shading net on the first few days (1-3 days) where 20.9 g m-3 (4.45% by weight) of astaxanthin was resulted. In addition, the various sizes of FP-APBRs exhibited similar performance implying a potential scale-up opportunity. In case of annual cost estimations of astaxanthin induction, results indicated that outdoor system charges were cheaper because this system did not require the use of fluorescent lamps as a light source for astaxanthin induction.
URL Website cuir.car.chula.ac.th
Chulalongkorn University

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