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Effects of dental radiation on the expression of apoptotic-related gene in primary human bone cells |
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| รหัสดีโอไอ | |
| Title | Effects of dental radiation on the expression of apoptotic-related gene in primary human bone cells |
| Creator | Sakarat Pramojanee |
| Contributor | Soontra Punmekiate, Prasit Pavasant |
| Publisher | Chulalongkorn University |
| Publication Year | 2551 |
| Keyword | Apoptosis, Cell, Cultured, Bone Marrow Cells, Humans, Gene Expression, Radiography, Dental, Radiography, Medical, Bone cells, X-rays -- Physiological effect, การบันทึกภาพด้วยรังสีทางการแพทย์, เซลล์กระดูก, รังสีเอกซ์ -- ผลกระทบทางสรีรวิทยา |
| Abstract | Exposure to 1 milligray ionizing radiation could induce DNA lesions probably leading to cell death through apoptotic process. Thus, performing dental radiography in a milligray range might be dangerous to human bone cells. The purpose of this study was to investigate the effects of dental radiation on the expression of apoptotic-related gene in primary human bone cells. Human bone cells were grown in 15% DMEM and irradiated with 0, 1, 2 doses of a periapical radiograph. The cytotoxicity of irradiation was investigated by MTT assay after 24 hours. The level of apoptotic-related gene expression (Bcl-2, Bax, Bad, Bcl-XL, Caspase-3) was analyzed by RT-PCR assay, four hours post irradiation No cytotoxicity was observed at 24 hours after dental irradiation. Interestingly, most of the results demonstrated a decrease of apoptotic-related gene expression at 4 hours after dental irradiation. Only Bax and Caspase-3 were significantly decreased compared to the control. In conclusion, dental radiation affects apoptotic-related gene expression. Therefore, prescribing dental radiograph should be highly selective and the principle of ALARA (As Low As Reasonably Achievable) should be followed. |
| URL Website | cuir.car.chula.ac.th |
