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Identification of genes involved in osmoregulation of the black tiger shrimp Penaeus monodon |
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| รหัสดีโอไอ | |
| Title | Identification of genes involved in osmoregulation of the black tiger shrimp Penaeus monodon |
| Creator | Suchonma Udomlertpreecha |
| Contributor | Anchalee Tassanakajon, Siriporn Pongsomboon |
| Publisher | Chulalongkorn University |
| Publication Year | 2549 |
| Keyword | Osmoregulation, Penaeus monodon, Penaeus monodon -- Genetics, Penaeus monodon -- Functional genomics, Functional genomics, Gene expression, การปรับระบบความดันออสโมติก, กุ้งกุลาดำ -- พันธุศาสตร์, กุ้งกุลาดำ -- การศึกษาหน้าที่ของยีน, การศึกษาหน้าที่ของยีน, การแสดงออกของยีน, กุ้งกุลาดำ |
| Abstract | Differential Display Polymerase Chain Reaction technique (DD-PCR) was used for the identification of genes involved in osmoregulation in antennal gland, epipodite and gill tissues of the salinity stressed Penaeus monodon. Shrimp reared at 25 ppt salinity as a control group were transferred to 3 and 40 ppt salinities for 6, 24 h and 2 weeks (stressed groups). Parallel comparison of stress and control DD-PCR profiles derived from 26 combinations of arbitrary and oligo-dT primer were performed to identify the differentially expressed genes. A total of 97 differentially expressed bands could be identified from 17 primer combinations. These bands were successfully reamplified, cloned and sequenced. A total of 129 unique sequences were found. Homology search showed that 37 different sequences significantly matched the GenBank database. Among the matched sequences, 22, 6 and 9 gene homologues were known genes, ribosomal proteins and hypothetical proteins, respectively. Twenty-three of 129 unique sequences including carbonic anhydrase, corin isoform 2, sarcolemmal associated protein 2, NIMA-family kinase Nek 7, karyopherin alpha 4, vacuolar protein sorting 18, CHK1 CHECKPOINT, Rps 16 protein, integrin alpha, 5 hypothetical proteins and 9 unknown gene products, were selected for confirmation of their differential expression patterns using semi-quantitative RT-PCR. The RT-PCR results confirmed that 21 out of 23 cDNA fragments were regulated by salinity stress. Eight cDNA fragments of unknown genes regulated by salinity from RT-PCR analysis were predicted for domain homologies using SMART program and Kyte-Doolittle hydropathy plots. The results showed that the encoded peptide of S5, S114 and S126 cDNA fragments contained transmembrane regions. In summary, the salinity-responsive genes that were identified from this study are potentially involved in osmoregulation of P. monodon. |
| URL Website | cuir.car.chula.ac.th |
