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Attachment of biotin on silicon surface-tethered poly(acrylic acid) brushes |
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| รหัสดีโอไอ | |
| Title | Attachment of biotin on silicon surface-tethered poly(acrylic acid) brushes |
| Creator | Piyaporn Akkahat |
| Contributor | Voravee P. Hoven |
| Publisher | Chulalongkorn University |
| Publication Year | 2548 |
| Keyword | Biotin, Polymers -- Surfaces, Polymerization |
| Abstract | Surface-tethered poly(acrylic acid) (PAA) brushed can potentially be used as functional nanometer-thick film for many technological applications. Their carboxyl groups serve as versatile moieties for a wide range of chemical modificantion including an attachment of biomolecules. In this research, linear and branched poly(tert-butyl acrylate) (PtBuA) brushed were prepared by surface-intiated atom transfer radical polymerization of tert-butyl acrylate and self-condensing vinyl copolymerization of PtBuA with acrylic acid 2-(2-bromopropionyloxy)ethyl ester (BPEA), respectively. Linear and branced PAA brushed were subsequently obtained after tert-butyl groups of PtBuA brushes were removed by acid hydrolysis. The carboxyl group density of linear and branched PAA brushes can be varied as a function of chain length (MW) and comonomer ration (grammar), respectively. The success of biotin attachment to the carboxyl groups of both linear and branched PAA brusheswas verified by FT-IR analysis and the binding with fluorescein-conjugated streptevidin which was visualized by fluorescence microscope. |
| ISBN | 9741419147 |
| URL Website | cuir.car.chula.ac.th |
