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A rapid fecal immunochemical test for gastrointestinal bleeding detection in cynomolgus macaques (Macaca fascicularis) |
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| รหัสดีโอไอ | |
| Creator | Suchinda Malaivijitnond |
| Title | A rapid fecal immunochemical test for gastrointestinal bleeding detection in cynomolgus macaques (Macaca fascicularis) |
| Contributor | Taratorn Kemthong, KrittigaSapkanarak, Piyachada Rounmakok, Thipchompoo Sing-ayudthaya, Lalitta Suriya-arunroj, Florian F?ger, Martin Werle |
| Publisher | Chulalongkorn University |
| Publication Year | 2564 |
| Journal Title | The Thai Journal of Veterinary Medicine |
| Journal Vol. | 51 |
| Journal No. | 2 |
| Page no. | 331 |
| Keyword | gastrointestinal bleeding, diarrhea, FIT, hemoglobin, cynomolgus macaque |
| URL Website | https://he01.tci-thaijo.org/index.php/tjvm/index |
| Website title | https://he01.tci-thaijo.org/index.php/tjvm/index |
| ISSN | 0125-6491 |
| Abstract | We used the Fecal ImmunochemicalOccult Blood Test (FIT) kit,a lateral flow immunochromatographic assayspecific for human hemoglobin, on cynomolgus monkeys (Macaca fascicularis). We aimed to employ this test to detect gastrointestinal injury in captive animals at the National Primate Research Center of Thailand-Chulalongkorn University (NPRCT-CU). The cross-reactivity and sensitivity of the test for monkey blood were determined and compared with the human blood. The anti-human hemoglobin antibody of the FIT kit reacted withthe monkey blood in a similarway as it did with humans and the intensity (T/C ratio) values between the two data sets closely correlated(R2 = 0.9324, p>0.05). Although the specificity for monkey blood was 4.2 times lower than for human blood,monkey blood with a dilution as low as 1:256,000could be detected in monkey fecal samples. Thus, we used the FIT kit to determine the gastrointestinal adverse effects of the NPRCT excipient which was orally administered daily to four female cynomolgus monkeys for 28 days. A daily visual inspection of freshly defecated fecal samples did not detect any blood but with the high sensitivity of the FIT kit the presence of hemoglobin in the feces was shown once in three of the four monkeys (3 of the 112 specimens determined). In conclusion, the human-FIT can be anapplicable tool for early detection of gastrointestinal injury in captive as well as wild cynomolgus monkeys. The advantage of this kit is that it requiresnon-invasivefecal sample collection, requires no additional equipment and gives results that can be read by the naked eye immediately. |
