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Possibility of trypsin treated dog erythrocytes as universal blood: evaluation by FTIR microspectroscopy |
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| รหัสดีโอไอ | |
| Creator | Pornphimon Metheenukul |
| Title | Possibility of trypsin treated dog erythrocytes as universal blood: evaluation by FTIR microspectroscopy |
| Contributor | KanjanaThumanu, Thanit Metheenukul |
| Publisher | Chulalongkorn University |
| Publication Year | 2564 |
| Journal Title | The Thai Journal of Veterinary Medicine |
| Journal Vol. | 51 |
| Journal No. | 2 |
| Page no. | 321 |
| Keyword | canine erythrocytes, dog erythrocyte antigen, DEA 1.1, FTIR spectromicroscopy, trypsin |
| URL Website | https://he01.tci-thaijo.org/index.php/tjvm/index |
| Website title | https://he01.tci-thaijo.org/index.php/tjvm/index |
| ISSN | 0125-6491 |
| Abstract | A common canine erythrocyte antigen is DEA 1.1 but it is the most immunogenic. In this study, Fourier transformed infrared (FTIR) microspectroscopywas used to examine the alterations of membrane biomolecules in trypsin-treated DEA 1.1+canine erythrocytes. Production of canine DEA 1.1-erythrocytes was achieved by treating DEA 1.1+red cells (5% cell suspension) with 1 mg/ml trypsin for 120 mins, without affecting the intracellular 2,3-diphosphoglycerate level. FTIR microspectroscopy was employed to study the effects of trypsin-treatment on canine trypsinized red cell membrane components. Production of canine DEA 1.1-erythrocytes was achieved without affecting the intracellular 2,3-diphosphoglycerate level. The FTIR microspectroscopy data suggested that the trypsin treated dog erythrocytes were possible for use as universal blood. |
