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The Comparison Effects of the Porcine Follicular Fluid to a Mixture of Follicle-Stimulating Hormone, Luteinizing Hormone and Estradiol Supplements on In vitro Porcine Oocyte Maturation |
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| รหัสดีโอไอ | |
| Creator | Mayuva Youngsabanant |
| Title | The Comparison Effects of the Porcine Follicular Fluid to a Mixture of Follicle-Stimulating Hormone, Luteinizing Hormone and Estradiol Supplements on In vitro Porcine Oocyte Maturation |
| Contributor | Nongnuch Gumlungpat, Sukjai Rattanayuvakorn |
| Publisher | Thammasat University |
| Publication Year | 2566 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 11 |
| Journal No. | 2 |
| Page no. | 22-36 |
| Keyword | Porcine follicular fluid, pig, in vitrooocyte maturation |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | The aim of this study wasto comparetheeffects of supplementingporcine follicular fluid(pFF) anda mixture offollicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol(E2) on in vitro porcine oocytes development. The pFF were collected from the Large Write Pigs' medium-sized follicle of 4-6 mm in diameter. oocytes at concentrations of 200 and 500 ?g protein/mL cultured in the 37 oC sterile-conditioned thermos incubator with 5% high humidity and 95% CO2in the atmosphere, supplemented by 2.2 mg/mL Medium 199, 0.25 mM NaHCO3, 15 ?g/mL and 50 ?g/mL pyruvate as well as gentamicin sulfate. The experiment was divided into 4 groups: Group 1, oocytes cultured in Medium199as a control group; Group 2, oocytes cultured in Medium 199 supplemented with LH, FSH and E2; Group 3, oocytes cultured in Medium 199 supplemented with LH, FSH,E2and 10% FBS; and Group 4, oocytes cultured in Medium 199 supplemented with pFF in 200 and 500 ?m protein/mL. We cultured for 24 and 44 h and measured the diameter of oocytewhich had sizing extension as growing.The results demonstrated that the pFF with a 200 ?g/mL concentration did not have impact on the growth of surrounding cells (). while that with a 500 ?g/mL concentration leaded to a significantly higher growth percentage than that of the positive control (supplemented by hormones and bovine serum), statistically(P<0.05). The surrounding cells extended and turned more fibroblastic than the positive control. Conclusively, the pFF from medium-sized oocytes with a 500 ?g protein/mL was the more appropriate supplement in the culture medium than those of costly hormones and BPS for enhancing the oocyte development. |
