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Optimal Conditions for Phytase Activity from Epiphytic Yeast Rhodotorula mucilaginosa ZML2-31and Their Properties for Phosphate Liberation |
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| รหัสดีโอไอ | |
| Creator | Ruthada Chanklan |
| Title | Optimal Conditions for Phytase Activity from Epiphytic Yeast Rhodotorula mucilaginosa ZML2-31and Their Properties for Phosphate Liberation |
| Contributor | Sasitorn Jindamorakot, Savitree Limtong |
| Publisher | Thammasat University |
| Publication Year | 2564 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 10 |
| Journal No. | 4 |
| Page no. | 388-401 |
| Keyword | phytase, phytate, phylloplane, epiphytic yeast, Rhodotorula mucilaginosa |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | Epiphytic yeast ZML2-31, isolated from corn phylloplane, exhibited high activity of 46.88 U/mL extracellular and 31.25 U/mL cell bound phytase when grown on LMM supplemented with 0.5 %Na-phytate at 24 and 48 h of cultivation, respectively. The optimal pH and temperature of crude phytase were pH 4.0 and 40?C, respectively. The residual phytase activities were more than 80% at 30-60 ?C for 2 h. Storage stability of phytase was retained more than 75% of the initial activity after 18 days at storage temperature ? 30 ?C. Addition of crude phytase to soybean and chickpea significantly enhanced inorganic phosphate liberation compared with control. Sequence analysis of D1/D2 domain of 26S rRNA gene revealed that the isolate ZML2-31 was highly related to Rhodotorula mucilaginosa with sequence identity of 99%. |
