Genetic Relationship Assessment of Indigofera Using Nucleotide Sequences of rpoC1 Gene and trnH-psbA Intergenic Spacer Region
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Creator Supharat Buaban
Title Genetic Relationship Assessment of Indigofera Using Nucleotide Sequences of rpoC1 Gene and trnH-psbA Intergenic Spacer Region
Contributor Nattapol Palawat, Theerachai Thanananta, Niramol Sakkayawong, Narumol Thanananta
Publisher Thammasat University
Publication Year 2563
Journal Title Thai Journal of Science and Technology
Journal Vol. 9
Journal No. 6
Page no. 844-853
Keyword Indigofera, genetic relationship, nucleotide sequence of specific region
URL Website https://www.tci-thaijo.org/
Website title THAIJO
ISSN 2286-7333
Abstract Indigofera, a plant in the Leguminosae, is an essential plant in fabric dyes production and has some pharmacological properties. This research aims to identify 15 Indigofera species using nucleotide sequences of rpoC1 genes and DNA fragments between trnH and psbA genes. Comparing nucleotide sequences data in NCBI database can identify Indigofera species and outgroup plants. When analyzed with the ClustalW program and a phylogenetic tree was constructed using UPGMA method by MEGA X program. All of the samples were classified into two groups. Sample No. 4 could be identified as Indigofera tinctoria and sample No. 13 (outgroup) could be identified as Vigna angularis. This research can be used as information for the conservation and improvement of Indigofera breeding.
Thai Journal of Science and Technology

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