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Genetic Relationship Assessment of Indigo Plant (Indigofera Linn.) Using SCoT Technique |
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| รหัสดีโอไอ | |
| Creator | Supharat Buaban |
| Title | Genetic Relationship Assessment of Indigo Plant (Indigofera Linn.) Using SCoT Technique |
| Contributor | Narumol Thanananta, Theerachai Thanananta |
| Publisher | Thammasat University |
| Publication Year | 2563 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 9 |
| Journal No. | 2 |
| Page no. | 333-341 |
| Keyword | indigo plant, Indigofera, genetic relationship, SCoT technique |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | Currently, Indigofera is popularly used as a raw material for the production of natural dyes because it is well known that indigo plants contain indigo substance. When exposed to air it is changed to indigo blues, which can give a navy-blue color in the weaving yarn. In addition, indigo plant is an herb to relieve swelling and pain from insect bites as well. The aim of this research was to evaluate the genetic relations of 15 Indigofera samples using start codon targeted (SCoT) technique. Eighty SCoT primers were screened, and 18 primers were able to be increased for DNA amplification. The total of 185 DNA bands appeared in sizes ranging from 200 to 2,850 base pairs, resulting in 5 monomorphism bands and 180 polymorphism bands (98 percent). A dendrogram using NTSYS-pc version 2.01e was constructed using unweighted pair group method with arithmetic mean (UPGMA). The coefficients of similarity were in a range of 0.46-0.88, and could separate 15 Indigofera samples into 7 groups. The results of this research can be used to plan for conservation and further breeding. |
