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การค้นหายีนในกลไกการออกดอกของมะพร้าว (Cocos nucifera L.) ด้วยเทคนิค Differentially Display RT-PCR |
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| รหัสดีโอไอ | |
| Creator | จรีรัตน์ มงคลศิริวัฒนา |
| Title | การค้นหายีนในกลไกการออกดอกของมะพร้าว (Cocos nucifera L.) ด้วยเทคนิค Differentially Display RT-PCR |
| Contributor | ศิริวรรณ ไทยสกุล, ปริยา มณีประเสริฐ |
| Publisher | Thammasat University |
| Publication Year | 2562 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 8 |
| Journal No. | 5 |
| Page no. | 532-543 |
| Keyword | coconut, flowering transition, prolong vegetative growth phase, DDRT PCR (differentially display RT-PCR) |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | To identify novel genes involved to control flowering transition of coconut, DDRT-PCR (differentially display RT PCR) was used to compare expression of genes in somatic apical meristem (SAM) during vegetative growth phase, Vg (germination, 4, 6, 12 and 24 months of age)and reproductive phase, Rp (36 months of age) of dwarf coconut.The result revealed that genes encoding Adenylate kinase, DNA (cytosine-5)-methyltransferase 1b, 14-3-3 protein 6, Retrotransposon RLC_Rider_Eg133H20-1, ALP1-like transposon and uncharacterized proteins, (LOC105038377 and LOC105032930) were identified as up-regulated genes in reproductive phase. These candidate genes were monitoredexpression during growth and development both in dwarf (early flowering type) and tall coconut (late flowering type) using quantitative Real time PCR. The expression of six those genes, except ALP1-like, showed gradually increase during vegetative growth phase (germination, 4, 6, 12 and 24 months) and then induced up-regulation when enter reproductive phase both in two types (36 months in dwarf and 60 months in tall). Interestingly, ALP1 like showed dynamic expression, up-regulated during seedling and then disappear during juvenile and up-regulated again during adult and reproductive phase. The different pattern between dwarf and tall coconuts was found. It showed delay expression in tall coconut. Taken its function and this result together, we proposed that shorten vegetative growth phase and early flowering of dwarf coconut was probably controlled by ALP1-like. |
