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การประยุกต์ใช้เครื่องหมายไอเอสเอสอาร์เพื่อตรวจสอบการเป็นลูกผสมข้ามชนิดระหว่างปทุมมาและกระเจียว |
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| รหัสดีโอไอ | |
| Creator | นฤมล เข็มกลัดเงิน |
| Title | การประยุกต์ใช้เครื่องหมายไอเอสเอสอาร์เพื่อตรวจสอบการเป็นลูกผสมข้ามชนิดระหว่างปทุมมาและกระเจียว |
| Contributor | สถาพร สุขจิตร, ช่อทิพา สกูลสิงหาโรจน์, เฉลิมศรี นนทสวัสดิ์ศรี |
| Publisher | Thammasat University |
| Publication Year | 2562 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 8 |
| Journal No. | 3 |
| Page no. | 287-299 |
| Keyword | Paracurcuma, Eucurcuma, interspecific hybrids and ISSR markers |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | Siam tulip, belonging to the family Zingiberaceae; genus Curcuma, is one of the economically important flowers in Thailand. To create novel hybrids attracting market demands, interspecific hybrids crossed between Siam tulip and its related species have been generated through sophisticated crossing techniques and embryo rescue. However, the hybridization process may create selfing or induce somatic embryogenesis. Hence, it is necessary to verify the true interspecific hybrids. The objective of this study was to apply Inter-Simple Sequence Repeat (ISSR) markers for detection of interspecific hybrids between Paracurcuma and Eucurcuma. The results of DNA amplification using 10 ISSR primers showed 72 amplified bands in total with 250-3,000 bp in size. Seventy-one amplified bands out of total (98.61%) were polymorphic. Moreover, we also found that 9 primers (90%) could be used for interspecific hybrid detection. A dendrogram based on polymorphic bands showed genetic similarities among parental species with similarity coefficients ranging between 0.04-0.57. In conclusion, the results in this study elucidated that ISSR markers were efficient to verify interspecific hybrids between Paracurcuma and Eucurcuma, which might be able to apply to other hybrids related to Curcuma sp.. Furthermore, the genetic relationship shown in this study might be useful in the curcuma breeding program. |
