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ความสัมพันธ์ทางพันธุกรรมของลิ้นจี่จากเครื่องหมายโมเลกุล ไอเอสเอสอาร์ ลำดับนิวคลีโอไทด์ของยีน rbcL และบริเวณระหว่างยีน trnL-trnF |
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| รหัสดีโอไอ | |
| Creator | ยุพเยาว์ คบพิมาย |
| Title | ความสัมพันธ์ทางพันธุกรรมของลิ้นจี่จากเครื่องหมายโมเลกุล ไอเอสเอสอาร์ ลำดับนิวคลีโอไทด์ของยีน rbcL และบริเวณระหว่างยีน trnL-trnF |
| Contributor | สิริมา สุวรัตน์, แสงทอง พงษ์เจริญกิต, วินัย วิริยะอลงกรณ์, จันทร์เพ็ญ สะระ, ศรัณย์ จีนะเจริญ |
| Publisher | Thammasat University |
| Publication Year | 2562 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 8 |
| Journal No. | 3 |
| Page no. | 271-286 |
| Keyword | Litchi chinensis, ISSR marker, intergenic spacer region of trnL-trnF, rbcL gene |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | Lychee (Litchi chinensis Sonn.) is an economically important fruit crop in Thailand, mainly cultivating in the northern and central part of the country. The popular cultivars are Hong Huai, Ohia, Kimjeng, Chakraphat and Khom. Currently, climate changes cause on-off flowering of lychee, and therefore many farmers have stopped growing the crop which may lead to loss of traditional cultivars. Meajo University thus have collected and conserved many lychee cultivars in the germplasm field. In establishing a genetics database, it is necessary to classify lychee cultivars using molecular markers. This research aimed to genetically identify 23 lychee cultivars using 20 ISSR (Inter-Simple Sequence Repeat) markers and DNA sequences of rbcL gene together with trnL-trnF intergenic spacer region. The results showed that ISSR markers produced 91 bands, 79 (86.81%) of which were polymorphic. The genetic relationship analysis revealed the similarity coefficients among lychee cultivars ranged from 0.529-1.000, and the lychee samples were categorized into 3 groups: group I consisted of cultivars grown in the central part of the country; group II consisted of cultivars from central and northern part and group III composed of those from the northern part. However, three cultivar pairs were not genetically separated. Analysis of DNA sequences of rbcL gene and trnL-trnF intergenic spacer region showed the genetic distance coefficients varied from 0.0000-0.0035, where Jeen Daeng cultivar was different from other cultivars. When analyzing genetic relationships using longan as an outgroup, lychee cultivars were separated from longan but among lychee cultivars could not be distinguished, except Jeen Daeng cultivar. |
