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Overexpression of OSB2 gene in transgenic rice up-regulated expression of structural genes in anthocyanin biosynthesis pathway |
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| รหัสดีโอไอ | |
| Creator | 1. Chotipa Sakulsingharoj 2. Poonsri Inta 3. Roypim Sukkasem 4. Saengtong Pongjaroenkit 5. Srimek Chowpongpang 6. Varaporn Sangtong1 |
| Title | Overexpression of OSB2 gene in transgenic rice up-regulated expression of structural genes in anthocyanin biosynthesis pathway |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2557 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 7 |
| Journal No. | 3 |
| Page no. | in press |
| Keyword | anthocyanin biosynthesis, OSB2 gene, Oryza sativa, transformation, Agrobacterium |
| ISSN | 24081310 |
| Abstract | Anthocyanin biosynthesis is regulated by regulatory genes encoding transcription factors which control expression of several structural genes in the anthocyanin pathway. The OSB2 gene encodes a Myc-type basic-helix-loop-helix (bHLH) transcription factor for anthocyanin biosynthesis in rice plants. We previously cloned the OSB2 gene from Thai black rice variety, Khum. In this study, the cloned OSB2 gene was transformed into white rice varieties Nipponbare and Taichung 65 to study the regulation of anthocyanin biosynthesis in rice. The OSB2 gene was constructed under dual 35s promoter and nos terminator for transformation of rice mediated by Agrobacterium. The transformed rice plants both Nipponbare and Taichung 65 had green tissues similar to wild-type plants, indicating no anthocyanin accumulation. However, semi-quantitative RT-PCR analysis revealed that transgenic rice plants overexpressing the OSB2 gene up-regulated the expression of some structural genes in anthocyanin biosynthesis including early biosynthesis gene (EBG) i.e. F3H (flavonoid-3-hydroxylase) and also late biosynthesis genes (LBGs) i.e. DFR (dihydroflavonol-4-reductase) and ANS (anthocyanin synthase). The present results support the other evidences that the regulatory mechanism of anthocyanin biosynthesis in rice may be different from other plants in which EBGs and LBGs were regulated separately. Overall results provide the understanding of functions of OSB2 gene in regulation of anthocyanin biosynthesis pathway in rice and will be useful to apply this gene as a visible marker gene for the improvement of rice varieties through genetic engineering in the future. |
