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การทำให้กุ้งกุลาดำติดเชื้อไวรัส Penaeus monodon densovirus ในห้องปฏิบัติการ (Experimental Infection of Black Tiger Shrimp with Penaeus monodon Densovirus (PmDNV) ) |
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| รหัสดีโอไอ | |
| Creator | ฉวีวรรณ ฉิมวัย |
| Title | การทำให้กุ้งกุลาดำติดเชื้อไวรัส Penaeus monodon densovirus ในห้องปฏิบัติการ (Experimental Infection of Black Tiger Shrimp with Penaeus monodon Densovirus (PmDNV) ) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 421 |
| Keyword | Penaeus monodon densovirus, PmDNV, HPV, experimental infection |
| ISSN | 8578664 |
| Abstract | Penaeus monodon densovirus (PmDNV) (formerly hepatopancreatic parvovirus or HPV) is one of the major causative pathogen of shrimp with a slow growth rate and stunted appearance in Thailand. The lack of available shrimp cell lines and limited studies on the experimental infection of PmDNV have hampered the study of this disease in shrimp. This study provides the evidence of PmDNV infection in P. monodon by injection through haemolymph. The black tiger shrimp (P. monodon) was injected with the PmDNV lysate extracted from hepatopancreas of PmDNV-infected shrimp. PCR and RT-PCR were used to analyse the viral infectivity. Treated shrimp were collected on Days 1, 7, and 14 post-inoculation. The considerably higher PmDNV was detected on Day 14 post-injection when compared with the first date after injection. It indicates a successful technique for experimental transmission of PmDNV in P.monodon. This work is supported by Thailand Research Fund and Mahidol University (DBG53-PA), the Office of the Higher Education Commission and Mahidol University under the National Research Universities Initiative and Mahidol University research grant. |
