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การใช้อาร์เอนเอสายคู่เพื่อควบคุมไวรัสแหลมสิงห์ในกุ้งกุลาดำ (Using Double-Stranded RNA for the Control of Laem-Singh Virus (LSNV) in Thai P.monodon) |
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| รหัสดีโอไอ | |
| Creator | วรรณวิมล ศักดิ์เสมอพรหม |
| Title | การใช้อาร์เอนเอสายคู่เพื่อควบคุมไวรัสแหลมสิงห์ในกุ้งกุลาดำ (Using Double-Stranded RNA for the Control of Laem-Singh Virus (LSNV) in Thai P.monodon) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 420 |
| Keyword | Laem-Singh virus, LSNV, dsRNA, feed, P.monodon shrimp |
| ISSN | 8578664 |
| Abstract | Development of specific and effective methods for controlling shrimp viral diseases is essential to lower the risk of severe economic losses. RNA interference (R NAi) is considered as a potential approach for inhibiting viral replication by introducing double-stranded (ds) RNA to interfere with viral mRNA. Laem-Singh Virus (LSNV) is a possible causative agent for monodon slow growth syndrome (MSGS) in Thai P.monodon. Oral administration of the LSNV-specific dsRNA was further tested in P.monodon for possible dsRNA application in farm operations. Feed formula was developed to meet dietary requirements and contain the LSNV-dsRNA. RT-PCR tests revealed 30% to 60% degree of LSNV inhibition in shrimp received feed containing dsRNA, and the results suggested that dsRNA inhibited the virus in a dose -dependent manner. For those with control feed, all tested shrimp were shown to be LSNV -positive. Average body weights of shrimp treated with dsRNA-feed were considerably higher than that of the control group. This study demonstrated for the first time that LSNV inhibition by oral application of virus -specific dsRNA could lead to the enhanced P.monodon growth, reaffirming the potential of dsRNA application for controlling viral disease in shrimp farm |
