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ประสิทธิภาพของสารสกัดจากพืชสมุนไพรไทยในการยับยั้งเชื้อยีสต์ที่ขาดยีนซึ่งเกี่ยวข้องกับการควบคุมการดื้อยา (The Antifungal Activity of Thai Herbal Extracts against Yeast Strains with Deletion of Drug Regulatory Genes) |
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| รหัสดีโอไอ | |
| Creator | สิริลักษณ์ บารมี |
| Title | ประสิทธิภาพของสารสกัดจากพืชสมุนไพรไทยในการยับยั้งเชื้อยีสต์ที่ขาดยีนซึ่งเกี่ยวข้องกับการควบคุมการดื้อยา (The Antifungal Activity of Thai Herbal Extracts against Yeast Strains with Deletion of Drug Regulatory Genes) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 183 |
| Keyword | Saccharomyces cerevisiae, Candida albicans, antifungal activity, essential oils, clove, cinnamon |
| ISSN | 8578664 |
| Abstract | Thai herbal extracts in the form of essential oils are used widely for various purposes such as in traditional medicine. Eventhough the antifungal properties of essential oils have been extensively studied; however, less is known about the mechanisms of inhibition. In this study, a solvent extraction using n-hexane is employed to obtain clove and cinnamon oils for examination of their antifungal activities against the human opportunistic pathogen Candida albicans and the model yeast Saccharomyces cerevisiae, using spot dilution assays. The results showed that the concentration of herbal oils required for inhibition of growth are 250 mg/ml of clove bud oil and 100 mg/ml of cinnamon bark oil for S. cerevisiae. For C. albicans, the minimum fungicidal concentration of clove and cinnamon oil was identified to be 200 and 100 mg/ml concentration, respectively. However, deletion of gene, which encodes for a regulatory protein, resulted in increased resistance to both essential oils in C. albicans. Thus, clove and cinnamon oil extracted by n-Hexane also has promising antifungal activities and may have potential use in the development of natural products as alternative antifungal agents. |
