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การศึกษาความหลากหลายทางพันธุกรรมของกล้วยสกุลมิวซาด้วยเทคนิคแฮตอาร์เอพีดี (Study on Genetic Diversity of Musa by Using High Annealing Temperature Random Amplified Polymorphic DNA Technique) |
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| รหัสดีโอไอ | |
| Creator | ฐิติพร โท้มโสภา |
| Title | การศึกษาความหลากหลายทางพันธุกรรมของกล้วยสกุลมิวซาด้วยเทคนิคแฮตอาร์เอพีดี (Study on Genetic Diversity of Musa by Using High Annealing Temperature Random Amplified Polymorphic DNA Technique) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 201 |
| Keyword | genetic diversity, Musa, HAT-RAPD |
| ISSN | 8578664 |
| Abstract | High annealing temperature random amplified polymorphic DNA (HAT-RAPD) technique was used to study the genetic diversity among 10 Musa cultivars. Seventy-two random primers were screened and 52 primers could be used for DNA amplification. Fifteen primers which gave clear amplified products were selected and used to analyze all cultivars. The result showed significant differences among 10 cultivars by using specific fragments for each cultivar. Moreover, 4 primers were able to identify all cultivars. A dendrogram, which constructed base on polymorphic bands using the NTSYS program, showed genetic similarities among the 10 species of Musa and separated to 4 clusters with similarity coefficients ranging from 0.50 to 0.89. |
