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การประยุกต์เทคนิคแฮตอาร์เอพีดีเพื่อตรวจสอบการกลายในถั่วเขียว (Applied HAT-RAPD Technique for Detection of Mutation in Mung Bean) |
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| รหัสดีโอไอ | |
| Creator | จาตุรงค์ สัมฤทธิ์ |
| Title | การประยุกต์เทคนิคแฮตอาร์เอพีดีเพื่อตรวจสอบการกลายในถั่วเขียว (Applied HAT-RAPD Technique for Detection of Mutation in Mung Bean) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 248 |
| Keyword | mung bean, HAT-RAPD, mutation |
| ISSN | 8578664 |
| Abstract | Mung bean [Vigna radiata (L.) Wilczek] is a legume used as a kind of food and dessert. It is native to the Indian subcontinent and can be grown during the three times a year, but prefered the late rainy season is strongly recommended. Thai breeders have been breeding in mung bean continuously. Today, the growing popularity of breed is Khampang Saen 2 (KPS2) which features a variety of high yield and resistance to leaf spot. Therefore, the mung bean seed also tested and irradiated at 200, 400, 500, 600, 700 and 800 Gy and then grown to check the efficiency of the mutant effect by using HAT-RAPD technique. In this study found that mung bean have been grown for 7 days, gamma radiation on the LD50is 678.43 Gy and HAT-RAPD technique show that mung bean at 400 Gy of gamma radiation have differences in DNA fingerprinting (A value close to LD30) was the gamma radiation levels that cause the most mutation. This suggests that HAT-RAPD technique can determine the efficiency of mutation in mung bean and applied to detect mutation in the other organisms. This is especially useful for research and breeding methods to induce. |
