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การประเมินความจาเพาะของไพรเมอร์ที่ใช้ตรวจ Salmonella enterica subspecies enterica (Evaluation of the Specificity of Published Primers for Detecting Salmonella entericasubspecies enterica) |
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| รหัสดีโอไอ | |
| Creator | อุไรวรรณ นาคเขียว |
| Title | การประเมินความจาเพาะของไพรเมอร์ที่ใช้ตรวจ Salmonella enterica subspecies enterica (Evaluation of the Specificity of Published Primers for Detecting Salmonella entericasubspecies enterica) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 290 |
| Keyword | Salmonella enterica subspecies enterica, PCR, specific primer |
| ISSN | 8578664 |
| Abstract | The present study were evaluated the specification of five published primers for detecting/identifying Salmonella enterica subspecies enterica strains by polymerase chain reaction (PCR) assay. The results demonstrated that ST11-ST15 primers showed 100% specification of amplification to 59 Salmonella enterica subspecies enterica strains in 22 serogroups, whereas primers of sipB-sipC, iroB, stn and hilA were 93.2%, 89.8%, 88.1% and 84.7%, respectively. The results from this study will be useful for further development of rapid detection of strains in Salmonella enterica subspecies enterica by PCR. |
