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ความผันแปรของลาดับนิวคลีโอไทด์บางส่วนของยีนโปรตีนตอบสนองต่อความร้อน OsHsfA2a และ OsHsfB2c จากข้าวไทย (Variation of Partial Coding Sequence of Heat Stress Transcription Factor Genes, OsHsfA2a and OsHsfB2c, from Thai Rice) |
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| รหัสดีโอไอ | |
| Creator | แสงทอง พงษ์เจริญกิต |
| Title | ความผันแปรของลาดับนิวคลีโอไทด์บางส่วนของยีนโปรตีนตอบสนองต่อความร้อน OsHsfA2a และ OsHsfB2c จากข้าวไทย (Variation of Partial Coding Sequence of Heat Stress Transcription Factor Genes, OsHsfA2a and OsHsfB2c, from Thai Rice) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 285 |
| Keyword | OsHsfA2a, OsHsfB2c, PCR, deletion, transversion |
| ISSN | 8578664 |
| Abstract | Heat stress transcription factors (Hsfs) are important proteins involved in plant response to heat stress. Expression studies of Hsfs gene showed the higher expression level of Oryza sativa heat stress transcription factor A2a (OsHsfA2a) and OsHsfB2c in high temperature. Therefore, these two genes were isolated from Thai rice by polymerase chain reaction (PCR) and used for biodiversity study. The nucleotide comparison and protein motif searching of partial coding sequence of OsHsfA2a gene revealed 12 bp deletion at N-terminus in Thai rice varieties which may not affect the function of this transcription factor. Whereas partial coding sequence OsHsfB2cgene comparison and motif searching revealed tranversion in Khao Dawk Mali 105 and Jow Haw which lower DNA binding motif score. This transversion may affect the function of OsHsfB2cprotein. The full length genes will be isolated for further study |
