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การตรวจ enterotoxigenic Escherichia coli ที่ก่อโรคอุจจาระร่วงในลูกสุกรด้วยวิธี polymerase chain reaction (Detection of Enterotoxigenic Escherichia coli in Diarrheal Piglets by Polymerase Chain Reaction) |
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| รหัสดีโอไอ | |
| Creator | รัตนา ภาชนนท์ |
| Title | การตรวจ enterotoxigenic Escherichia coli ที่ก่อโรคอุจจาระร่วงในลูกสุกรด้วยวิธี polymerase chain reaction (Detection of Enterotoxigenic Escherichia coli in Diarrheal Piglets by Polymerase Chain Reaction) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 317 |
| Keyword | enterotoxigenic Escherichia coli, piglet, PCR |
| ISSN | 8578664 |
| Abstract | We detected virulence factors namely lt (heat labile), stp (heat stable in porcine) and sth (heat stable in human) of the enterotoxigenic Escherichia coli (ETEC) by polymerase chain reaction (PCR). A total of 100 rectal swabs from diarrheal piglets were collected. As a result, 98 E. coli strains, 38 strains from suckling and 60 strains from weaning piglets were isolated from these samples. Furthermore, 11.2% of total E. coli strains were ETEC. The ETEC strains harboring stp (13.4%) were found only in weaning piglets, whereas the strains harboring lt were found in both in suckling (2.6%) and weaning (3.3%) piglets, respectively. The findings from this study are necessary for surveillance of diarrheal disease outbreaks in piglets in swine farms. |
