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การตรวจ Vibrio parahaemolyticus สายพันธุ์ก่อโรคที่แยกได้จากตัวอย่างทางการแพทย์และสิ่งแวดล้อมด้วยวิธี Multiplex polymerase chain reaction (Detection of Pathogenic Vibrio Parahaemolyticus Isolated from Clinical and Environmental Samples by Multiplex |
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| รหัสดีโอไอ | |
| Creator | ณัฎฐา สุขตัว |
| Title | การตรวจ Vibrio parahaemolyticus สายพันธุ์ก่อโรคที่แยกได้จากตัวอย่างทางการแพทย์และสิ่งแวดล้อมด้วยวิธี Multiplex polymerase chain reaction (Detection of Pathogenic Vibrio Parahaemolyticus Isolated from Clinical and Environmental Samples by Multiplex Polymerase Chain Reaction) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 321 |
| Keyword | ldh, tdh, trh, multiplex PCR |
| ISSN | 8578664 |
| Abstract | In this study, we analyzed virulence genes; tdh and trh in Vibrio parahaemolyticus (VP) strains from clinical and environmental samples using multiplex polymerase chain reaction. A total of 100 VP strains were obtained from diarrheal patients (n=16), human carriers (n=19), blood cockles (n=49), and Asian green mussels (n=16). The result showed that 17% of strains were determined as pathogenic strains and 83% were non-pathogenic strains. The pathogenic strains with tdh + trh -were found at the highest prevalence (10%) followed by tdh -trh + (5%), and tdh + trh + (2%). Pathogenic strains were predominantly isolated from diarrheal patients (10%) virulence gene profiles of these isolates were tdh + trh -(37.5%), tdh -trh + (12.5%), and tdh + trh + (12.5%). The strains isolated from human carriers were positive for only tdh + trh -(21%), and tdh -trh + (5.3%). Conversely, pathogenic strains from food samples (blood cockles and Asian green mussels) possessed only tdh -trh + in the lowest percent (3%). |
