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การโคลนและการหาลาดับนิวคลีโอไทด์บางส่วนของยีน cyp19a ในปลากะพงขาว (Lates calcarifer) (Cloning and Partial Sequencing of cyp19a of Sea Bass (Lates calcarifer)) |
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| รหัสดีโอไอ | |
| Creator | พิจิตรา ศรีพัดยศ |
| Title | การโคลนและการหาลาดับนิวคลีโอไทด์บางส่วนของยีน cyp19a ในปลากะพงขาว (Lates calcarifer) (Cloning and Partial Sequencing of cyp19a of Sea Bass (Lates calcarifer)) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 361 |
| Keyword | cyp19a, cytochrome P450 aromatase A, Lates calcarifer/ sea bass |
| ISSN | 8578664 |
| Abstract | Cloning and partial sequencing of cyp19a (Cytochrome P450 Aromatase A) of sea bass (Lates calcarifer) were studied. DNA was extracted from liver tissue of adult sea bass and cyp19a was then amplified by PCR (Polymerase Chain Reaction). The cyp19a amplicons were cloned and subsequently sequenced revealing that the obtained cyp19a gene was 629 bp in length. The partial cyp19a gene structure comprises 3 exons (90, 154 and 162 bp, respectively) and 2 introns (96 and 127 bp, respectively). The amplified exonic DNA regions compared to the GenBank database revealed highly similarity (92% identity) with the cyp19a mRNA of Dicentrachus labrax (GenBank accession no. AJ311177; retrieved on December 25, 2012). Analysis of the cyp19a and those of the other teleosts by phylogenetic tree confirmed that the amplified DNA fragment is cyp19a. The result from this study provides information that will lead in further analysis of the complete cyp19a gene structure. The cyp19a gene segment can also be applied to use as a molecular biomarker for screening of the estrogenic compounds and polycyclic aromatic hydrocarbons contamination in the aquatic environment. |
