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การระบุเพศนกในสกุลนกหัวโตเล็ก (Sex Identification of Birds in Genus Charadrius) |
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| รหัสดีโอไอ | |
| Creator | ณิชาภัทร ชอบอาภรณ์ |
| Title | การระบุเพศนกในสกุลนกหัวโตเล็ก (Sex Identification of Birds in Genus Charadrius) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 383 |
| Keyword | sex identification, chromo-helicase-DNA binding (CHD) gene, Charadrius sp |
| ISSN | 8578664 |
| Abstract | Charadrius spp. are small shorebirds which wintering in Thailand during non-breeding period. outside the breeding season the adult is nearly sexually monomorphic that sex is difficult to identify. Therefore, sex identification using molecular technique is recommended. In this study, using FTA ? card, genomic DNA was collected from blood samples of three Charadrius spp., including lesser sand plover (Charadrius mongolus), greater sand plover (C. leschenaultii) and kentish plover (C. alexandrinus). The 2550F/2718R primer can be used to amplify the intronic region of the chromo-helicase-DNA binding (CHD) gene and the resulting PCR products were analyzed by agarose gel electrophoresis. The females produced two bands, which are 450 bp (CHD-W) and around 650 bp (CHD-Z), whereas samples from the males produced only a single band at around 650 bp (CHD-Z). Additionally, the nucleotides sequence of CHD-Z of C. alexandrines is different from C. mongolus and C. leschenaultii is 13 bp |
