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การโคลนและวิเคราะห์ลาดับนิวคลีโอไทด์บางส่วนของยีนไวเทลโลจีนิน (vtg) ของ ปลากะพงขาว (Lates calcarifer) (Cloning and Partial Sequencing of Vitellogenin Gene (vtg) of Sea Bass (Lates calcarifer)) |
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| รหัสดีโอไอ | |
| Creator | ศศิธร ฝอยหิรัญ |
| Title | การโคลนและวิเคราะห์ลาดับนิวคลีโอไทด์บางส่วนของยีนไวเทลโลจีนิน (vtg) ของ ปลากะพงขาว (Lates calcarifer) (Cloning and Partial Sequencing of Vitellogenin Gene (vtg) of Sea Bass (Lates calcarifer)) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 365 |
| Keyword | Lates calcarifer, vitellogenin, vtg |
| ISSN | 8578664 |
| Abstract | The aim of the present work was to study partial nucleotide sequences and structure of the amplified vitellogenin gene (vtg) of sea bass (Lates calcarifer) by Polymerase Chain Reaction (PCR). The vtg amplicons were cloned and then sequenced revealing that the obtained vtg gene was 733 bp in length. The partial vtg gene structure comprises 4 exons and 3 introns compared to the complete vtg gene of the rainbow trout (Oncorhynchus mykiss). The amplified exonic DNA regions were correlately revealed in exons 29, 30, 31 and 32 of the rainbow trout vtg gene. The sea bass L. calcarifer vtg sequences showed highly similarity (90% identity (360/400; E-value = 5e-52)) with the vitellogenin Ab mRNA of the European sea bass, Dicentrarchus labrax (GenBank accession no. JQ283442; retrieved on February 25, 2013). The vtg gene of sea bass was later confirmed by phylogenetic tree showing the obtained gene is vtg B. This study can be applied to improve fish breeding and propagation of cultivated fish and gene expression studies. The vtg gene can be used as a genetic biomarker for early screening of the estrogenic Endocrine Disrupting Chemicals (e-EDCs) contamination in the aquatic environment. |
