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การตรวจดีเอ็นเออย่างเร็วจากการเปลี่ยนสีบนพื้นฐานการทำนิวคลิอิคแอซิดไฮบริไดซ์เซชันในคอลลอยด์ผลึกเงินนาโนสีฟ้า (Rapid Colorimetric DNA Detection Based on Nucleic Acid Hybridization in Colloid Blue Silver Nanoplates) |
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| รหัสดีโอไอ | |
| Creator | ปิยะศักดิ์ ชอุ่มพฤกษ์ |
| Title | การตรวจดีเอ็นเออย่างเร็วจากการเปลี่ยนสีบนพื้นฐานการทำนิวคลิอิคแอซิดไฮบริไดซ์เซชันในคอลลอยด์ผลึกเงินนาโนสีฟ้า (Rapid Colorimetric DNA Detection Based on Nucleic Acid Hybridization in Colloid Blue Silver Nanoplates) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 387 |
| Keyword | colorimetric detection, DNA hybridization, silver nanoplates, plasmon |
| ISSN | 8578664 |
| Abstract | Novel technique for target DNA detection, based on the hybridization between DNA probe and target complementary DNA via nucleic acid hybridization in colloid of blue silver nanoplates was developed to induce plasmonic changes of the nanoplates. When the test was absence of the target DNA or having non target DNA, oligonucleotide probe did not react with DNA allowing hydrophobic portions of probe to bind to surface of silver nanoplates. Induction via adding salt in the test system made those nanoplates aggregated and had plasmonic change of color from blue to clear color. In contrast, when there was target DNAs in the system, oligonucleotide probe did react with target DNA and formed double stranded conformation protecting nanoplates from aggregation. This result is no colorimetric change. The binding of probe to target was specific and happened in 10 to 15 min which visible with naked eye. |
