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ยีน BADH2 มีความสัมพันธ์กับความหอมในข้าวฟ่าง (Sorghum bicolor (L.) Moench) (BADH2 Gene Associated with Fragrance in Sorghum (Sorghum bicolor (L.) Moench)) |
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| รหัสดีโอไอ | |
| Creator | ชุตินธร หยุนแดง |
| Title | ยีน BADH2 มีความสัมพันธ์กับความหอมในข้าวฟ่าง (Sorghum bicolor (L.) Moench) (BADH2 Gene Associated with Fragrance in Sorghum (Sorghum bicolor (L.) Moench)) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 410 |
| Keyword | sorghum, fragrance, aroma, mutation, 2AP, BADH2 |
| ISSN | 8578664 |
| Abstract | Pandan-like fragrance in several plants is due to the chemical 2-acetyl-1-pyrroline (2AP). The gene(s) responsible for fragrance in rice, sorghum and vegetable soybean encode non- or mal-functional betaine aldehyde dehydrogenase 2 (BADH2) resulting in production of 2AP. In this study, we reported an association between the BADH2 gene and fragrance in sorghum. An F 2 population developed from a cross between KEP-477 (fragrant) and KU630 (non-fragrant) was used. Leaves of F 2 and F 3 progenies were evaluated for fragrance by sensory test, while seeds of F 2 plants were analyzed for 2AP. The tests consistently showed that the fragrance is controlled by a single recessive gene. Gene expression analysis of BADH2 in leaves of KU630 and KEP-477 at various stages revealed that BADH2 was expressed normally in KU630 but low or nil in KEP-477. Sequence comparison of BADH2 between KU630 and KEP-477 revealed a continuous 1,444-bp deletion in KEP-477. Bulk-segregant analysis and quantitative trait locus mapping showed that BADH2 associated with fragrance evaluated by sensory test and 2AP analysis. A gene-based maker was developed for detecting the fragrance gene which will be useful for marker-assisted selection of fragrant sorghum(s) in breeding programs. |
