|
Multiplex PCR เหมาะสาหรับการศึกษาระบาดวิทยาของไรฝุ่น (Multiplex PCR is Superb for Epidemiological Survey on Dust Mite Infestation) |
|---|---|
| รหัสดีโอไอ | |
| Creator | ณัฐ มาลัยนวล |
| Title | Multiplex PCR เหมาะสาหรับการศึกษาระบาดวิทยาของไรฝุ่น (Multiplex PCR is Superb for Epidemiological Survey on Dust Mite Infestation) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 418 |
| Keyword | multiplex PCR, dust mite, identification |
| ISSN | 8578664 |
| Abstract | Since the conventional microscopic technique requires the skillful specialists and prolonged duration for dust mite identification, the multiplex PCR has been developed for identifying the three common dust mite species; Dermatophagoides pteronyssinus (Dpt), D. farinae (Df) and Blomia tropicalis (Bt). The preliminary investigation showed 100% specificity and the maximum sensitivity of the assay down to 10 ng of mite gDNA (less than a mite). Therefore, the assay has been further evaluated with the dust samples collected from houses in Thailand. Thirteen (30.23%) out of 43 dust samples gave the positive identification of either Dpt, Df or Bt mites by multiplex PCR, whereas 14 (32.56%) out of 43 samples was also positive by microscopic method. About 70% of samples gave the same results in both the multiplex PCR and the microscopic identification. Moreover, the multiplex PCR could detect the dust mites in the samples of which the mites were not found by the conventional method. Nevertheless, further evaluation is carrying on with more additional dust samples (N = 100). According to its less requirements on time consuming and acarological skillful, and its practical processes for large number of samples, it could preliminary be concluded that the multiplex PCR is a superb method for using in epidemiological study on dust mite infestation |
