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การตรวจสอบความหอมในเชื้อพันธุกรรมข้าวไร่ไทยโดยใช้เครื่องหมายดีเอ็นเอ ที่จำเพาะต่อยีน Os2AP และการวิเคราะห์คุณภาพหุงต้มและความหนาแน่นของธาตุเหล็กในเมล็ด |
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| รหัสดีโอไอ | |
| Creator | 1. ไวพจน์ กันจู 2. สุกัญญา เรืองขำ 3. สุมน ห้อยมาลา 4. อนุชา พลับพลา 5. อภิชาติ วรรณวิจิตร 6. ธีรยุทธ ตู้จินดา |
| Title | การตรวจสอบความหอมในเชื้อพันธุกรรมข้าวไร่ไทยโดยใช้เครื่องหมายดีเอ็นเอ ที่จำเพาะต่อยีน Os2AP และการวิเคราะห์คุณภาพหุงต้มและความหนาแน่นของธาตุเหล็กในเมล็ด |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 6 |
| Journal No. | 1 |
| Page no. | 12-24 |
| Keyword | aroma, upland rice, DNA marker,Os2AP gene, amylose content, iron |
| ISSN | 8578664 |
| Abstract | The aroma of rice is an important trait for its economic value. It is regulated by the Os2AP gene, which is located on the long arm of chromosome 8. The objective of this research is identification of aroma in 618 samples of Thai upland rice germplasm using 4 DNA markers located specifically on the Os2AP gene: Aromarker, 5'SSR, 3In2AP and 3'inde-UTR. Cooking qualities and iron content were also analyzed in rice seeds. The results showed that the Aromarker can be used to identify aromatic rice from all samples. A total of 78 samples of aromatic upland rice were detected by Aromarker including 57 samples of glutinous rice, 20 samples of non-glutinous rice and one sample of heterozygous genotype. For the 3In2AP marker, the aroma in upland rice germplasm cannot be identified because it showed a monomorphic DNA pattern. In addition, one of the aromatic upland rice displayed non-aromatic genotype at Aromarker and 3In2AP marker, but the 2-acetyl-1-pyrroline (2AP) content in brown rice seeds analyzed by Headspace-GC/MS was 2.52 ppm. Os2AP allele diversity in upland rice germplasm was evaluated using 2 DNA markers. The results showed that 3 alleles of 3'indel-UTR and 9 alleles of 5'SSR were detected in this population. Cooking qualities of rice grain were evaluated by amylose content (AC) and gelatinization temperature (GT). A total of 223 samples showed low amylose content between 10-19%. Most of them exhibited a GT value below 70oC. Perls' Prussian blue method was used to evaluate iron content in brown rice seeds. A total of 334 seed samples displayed high iron content at level 2, and 53 seed samples showed highest iron content at level 3. This study presented the high genetic variation and economic values of upland rice germplasm in Thailand. These rice germplasm can be distributed to farmer on highland areas, which will increase the genetic diversity of the cultivated rice. |
