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ความแปรปรวนทางพันธุกรรมในประชากรปลายี่สกเทศของไทยที่มาจากการเพาะเลี้ยง ปรับปรุงพันธุ์ และแหล่งน้ำธรรมชาติ |
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| รหัสดีโอไอ | |
| Creator | Panom Krachangpoj Sodsuk |
| Title | ความแปรปรวนทางพันธุกรรมในประชากรปลายี่สกเทศของไทยที่มาจากการเพาะเลี้ยง ปรับปรุงพันธุ์ และแหล่งน้ำธรรมชาติ |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 6 |
| Journal No. | 2 |
| Page no. | 150-167 |
| Keyword | Rohu (Labeo rohita), genetic variation, microsatellite |
| ISSN | 8578664 |
| Abstract | Genetic variation based on microsatellite markers was analysed among cultured, selectively bred and natural populations of rohu, Labeo rohita (Hamilton, 1822) in Thailand. Eight populations were used in the analysis which include a cultured population from Kalasin Inland Fisheries Research and Development Center, four populations (P0, F1, F2 and F3) from a selective breeding program of Uttaradit Aquaculture Genetics Research and Development Center and the other three natural populations from Mun River (Ubon Ratchathani Province), Bueng Si Fai (Phichit Province) and Moei River (Tak Province). With eight microsateliite loci screened, the genetic variabilities including per locus averages of number of alleles (Aa = 2.1250 3.2500), effective number of alleles (Ae = 1.6342 2.2075), allelic richness (Ar = 2.0696 2.7714), observed heterozygosity (Ho = 0.3183 0.5303) and expected heterozygosity (He = 0.3209 0.5031) were obtained, and were not significantly different among all the eight populations (P>0.05). Significant deviations from Hardy-Weinberg equilibrium were not found in any of the populations (PHWE>0.05). Estimated effective population sizes (Ne) were 1.5 109.5, of which the Kalasin Inland Fisheries Research and Development Center population was of the lowest Ne, so, particular attention should be paid to this population. Population differences analyses implied that the eight populations generally were in a single group, with genetic differences between the populations within this group. It was found from further analyses in each pair of populations that the populations of Kalasin Center, Mun River, Bueng Si Fai and Moei River were not different from each other (PFst>0.0007, Bonferroni correction). This finding also supported that these populations are related to those introduced to Thailand in 1968-1969. Another relative analysis of genetic structure, resulting in a close relationship among the P0, F1, F2 and F3 populations of the Uttaradit Center, confirmed that the P0, F1, F2 and F3 populations originated from the population introduced to Thailand in 1998. Results from the between-generations comparisons of P0, F1, F2 and F3 based on the population genetic data in this study and growth performance from the previous study showed that mass selective breeding by the Uttaradit Center could make better growth appear in the F2 generation, whereas the population genetic differentiation would begin to be affected in the F3 generation. Consequently, a recommendation is that much attention on genetic management should be paid to the brood-stocks after the F3 selectively bred generation has been achieved. |
