|
ลักษณะแฮพโพลไทป์ของยีนบีตาโกลบินของฮีโมโกลบินผิดปกติที่พบไม่บ่อยในภาคใต้ประเทศไทย (Beta Globin Gene Haplotype Analysis of Rare Hemoglobin Variants in Southern Thailand) |
|---|---|
| รหัสดีโอไอ | |
| Creator | วรรณรัตน์ แซ่ชั่น |
| Title | ลักษณะแฮพโพลไทป์ของยีนบีตาโกลบินของฮีโมโกลบินผิดปกติที่พบไม่บ่อยในภาคใต้ประเทศไทย (Beta Globin Gene Haplotype Analysis of Rare Hemoglobin Variants in Southern Thailand) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 127-129 |
| Keyword | hemoglobin variants, beta globin gene haplotype, PCR-RFLP |
| ISSN | 8578664 |
| Abstract | Hemoglobin variant is an inherited disorder that results in the abnormal structure of globinchains of the hemoglobin (Hb) molecule. Many hemoglobin variants have been characterized worldwide and the distribution of hemoglobin variants in southern Thailand is associated with geographic and/or ethnic backgrounds. The aim of this study was to investigate the genetic origin of rare hemoglobin variants in Thailand by characterized the beta-globin gene cluster haplotype pattern in Hb C 21 cases, Hb D-Punjab 14 cases, Hb Tak 4 cases and Hb G-Makassar 4 cases. Haplotype analysis was done by Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) for beta globin gene cluster of the following polymorphic restriction sites: HincII 5'?, XmnI 5'G?, HindIIIG?, HindIIIA?, HincII 5'??, HincII 3'?? and AvaII 5'?. This study found that the type of haplotype of hemoglobin variants in southern Thailand is apparently different from previously report. The haplotypes of Hb G-Makassar and Hb Tak were reported for the first time in southern Thailand. This finding suggests that the history of population admixture might contribute to the present distribution pattern of the haplotype variants. |
