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การศึกษาความหลากหลายทางพันธุกรรมและโครงสร้างประชากรของ Vigna exilis และ Vigna grandiflora โดยใช้เครื่องหมายไมโครแซทเทลไลท์ (Genetic Diversity and Population Structure of Vigna exilis and Vigna grandiflora based on Microsatellite Variation) |
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| รหัสดีโอไอ | |
| Creator | อโนชา แก้ววงษ์วาลย์ |
| Title | การศึกษาความหลากหลายทางพันธุกรรมและโครงสร้างประชากรของ Vigna exilis และ Vigna grandiflora โดยใช้เครื่องหมายไมโครแซทเทลไลท์ (Genetic Diversity and Population Structure of Vigna exilis and Vigna grandiflora based on Microsatellite Variation) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 415 |
| Keyword | azuki bean, microsatellite marker, mung bean, population genetics, SSR |
| ISSN | 8578664 |
| Abstract | The objective of this research was to determine genetic diversity and population structure of natural populations of two rare wild species of Asian Vigna (genus Vigna subgenus Ceratotropis), viz. Vigna exilis Tateishi and Maxted and Vigna grandiflora (Prain) Tateishi and Maxted from Thailand. Using 21 simple sequence repeat (SSR) markers, 107 and 85 individuals from 7 and 5 natural populations of V. exilis and V. grandiflora, respectively, were analyzed. In total, the markers detected 196 alleles for V. exilis and 219 alleles for V. grandiflora. V. exilis populations showed lower average values in number of alleles, allelic richness, observed heterozygosity, gene diversity, and outcrossing rate than V. grandiflora populations, being 58.00 vs. 114.60, 51.96 vs. 74.80, 0.02 vs. 0.18, 0.40 vs. 0.66, and 3.24% vs. 17.41%, respectively. Pairwise FST among populations demonstrated that V. exilis are much more differentiated than V. grandiflora which is supported by AMOVA analysis that 57.51 and 82.63 % of total variation resided within the populations of V. exilis and V. grandiflora, respectively. Seven and two genetic clusters were detected for V. grandiflora and V. exilis by STRUCTURE analysis. Our findings suggest that different strategies are needed for in situ conservation of the two species. All or as many as possible V. exilis populations should be conserved to protect genetic resources of this species. While a few V. grandiflora populations already capture the majority of its genetic variation in Thailand. |
