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การพัฒนาเครื่องหมาย SCAR เพื่อใช้ในการจาแนกลาไยลูกผสมระหว่างพันธุ์ดอ 27 กับสีชมพู (Development of SCAR (Sequence Characterized Amplified Region) Marker for Identification of F1Hybrid Longan (Dimocarpus lonyan Lour.) 'Do 27 X Si- Chomphu) |
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| รหัสดีโอไอ | |
| Creator | จันทร์เพ็ญ สะระ |
| Title | การพัฒนาเครื่องหมาย SCAR เพื่อใช้ในการจาแนกลาไยลูกผสมระหว่างพันธุ์ดอ 27 กับสีชมพู (Development of SCAR (Sequence Characterized Amplified Region) Marker for Identification of F1Hybrid Longan (Dimocarpus lonyan Lour.) 'Do 27 X Si- Chomphu) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 244-247 |
| Keyword | longan, SCAR marker, F1 hybrid longan |
| ISSN | 8578664 |
| Abstract | Longan (Dimocarpus longan Lour.) is commercially important crop in the northern part of Thailand. 'Do' variety has been popular, mostly grown about 79 % of growing areas. This causes low genetic diversity of longan. However, breeding program of longan typically takes a long time. Therefor, molecular markers could be developed to select F1hybrid. This study would develop SCAR marker for selection of F1hybrid of longan 'Do 27 X Si-Chomphu'. Fifty RAPD primers were screened and two primers (N-04 and BPS 5) showed polymorphic bands from Si-Chomphu. These bands were purified and cloned into the vector. Recombinant clones were sequenced. The DNA sequences were used for desining primer, which were specific to Si-Chomphu. The PCR products of these 2 primer pairs were 200 bp bands were used as the SCAR markers. These SCAR markers could distingnish Si-Chomphu and F1hybrid from Do 27 x Si-Chomphu |
