|
การวิเคราะห์ลาดับนิวคลีโอไทด์ของยีนทนแล้ง (EgLEA4) จากปาล์มน้ามัน (Elaeis guineensis Jacq) (Sequence Analysis of Drought-tolerance (EgLEA4) Gene from Oil Palm (Elaeisguineensis Jacq)) |
|---|---|
| รหัสดีโอไอ | |
| Creator | วลีรัตน์ ทองอินทร์ |
| Title | การวิเคราะห์ลาดับนิวคลีโอไทด์ของยีนทนแล้ง (EgLEA4) จากปาล์มน้ามัน (Elaeis guineensis Jacq) (Sequence Analysis of Drought-tolerance (EgLEA4) Gene from Oil Palm (Elaeisguineensis Jacq)) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 266-269 |
| Keyword | late embryogenesis abundant protein, drought, oil palm, EgLEA4 |
| ISSN | 8578664 |
| Abstract | The late embryogenesis abundant (LEA) proteins in oil palm have been implicated as part of the stress response especially those associated with drought in plants. We first isolated the EgLEA4 gene from an EST library of oil palm (Elaeis guineensis Jacq.). The structural gene of EgLEA4 consisted of 1505 bp containing 2 exons (108 and 378 bp), 1 intron (313 bp), and the promoter region of an EgLEA4 (447 bp). The 5' untranslated region showed a putative promoter sequence such as an ABA-responsive element (ABRE) and a salt/drought/dehydration responsive element (DRE). The protein sequence analysis showed that it consisted of 50.3% hydrophilic residues and 49.7% hydrophobic residues. Hydropathicity analysis predicted that this protein was a hydrophilic protein. The amino acid patterns of EgLEA4 consisted of five conserved motifs (II, I, III, IV and V). The high expression of the EgLEA4 gene in the mesocarp tissue and the highest expression in the late stage of oil palm fruits development were associated with the decreased water content of the fruit's tissue. |
