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การโคลนและศึกษายีน Stearoyl ACP desaturase (SAD) ในปาล์มน้ามัน (Elaeis guineensis Jacq.) (Cloning and Characterization of Stearoyl ACP Desaturase Gene (SAD) in Oil Palm (Elaeis guineensis Jacq.)) |
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| รหัสดีโอไอ | |
| Creator | วรินทร์ทิพย์ ครุธแก้ว |
| Title | การโคลนและศึกษายีน Stearoyl ACP desaturase (SAD) ในปาล์มน้ามัน (Elaeis guineensis Jacq.) (Cloning and Characterization of Stearoyl ACP Desaturase Gene (SAD) in Oil Palm (Elaeis guineensis Jacq.)) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 394 |
| Keyword | oil palm, fatty acid, SAD (Stearyl-ACP desaturase) |
| ISSN | 8578664 |
| Abstract | The Oil palm (Elaeis guineensis Jacq.) is the highest producing oil seed crop on a per hectare basis in the world. Current estimates are that within the next decade, palm oil will become the largest single oil traded globally. This study has thoroughly conducted the research on cloning and characterization of Stearyl ACP desaturase gene (SAD) in oil palm. Mesocarp tissue of oil palm fruit at different developmental stages including 0, 30, 60, 90, 120, 150, and 180 days after pollination (DAP) was used for expression pattern analysis. The results showed that SAD gene was highly expressed at 90 DAP. SAD gene is 1,179 base pair in length and has 3 exons and 2 introns. It is translated into 393 amino acids. The phylogenetic analysis was constructed using nucleotide sequences and it is closely related to SAD gene from Elaeis oleifera |