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Association study of the MMP1 gene polymorphism with knee osteoarthritis in Thais by high resolution melting (HRM) analysis |
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| รหัสดีโอไอ | |
| Creator | 1. Thitiya Poonpet 2. Sittisak Honsawek 3. Nattapol Tammachote 4. Supakit Kanitnate 5. Rachaneekorn Tammachote |
| Title | Association study of the MMP1 gene polymorphism with knee osteoarthritis in Thais by high resolution melting (HRM) analysis |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 6 |
| Journal No. | 2 |
| Page no. | 181 |
| Keyword | Osteoarthritis, MMP1, SNP, rs1799750, HRM analysis, Thais |
| ISSN | 8578664 |
| Abstract | Osteoarthritis (OA) is the most common chronic joint disease in aging population. It is characterized by degeneration and loss of cartilage at the joint surface, accompanied by subchondral bone changes. Since an important characteristic of OA is the loss of collagen network, matrix metallo proteinases (MMPs) with collagenase activity are believed to participate as a possible cause of OA. This work aimed to investigate the association between a deletion SNP in MMP1 promoter (rs1799750; 2G/1G) and knee OA susceptibility in Thai population. The study subjects consisted of 108 knee OA patients and 119 healthy controls. SNP genotyping was carried out by real-time PCR based high resolution melting (HRM) analysis, and the melting profiles from all DNA samples were analysed with Precision Melt AnalysisTM software. The allele frequencies (2G and 1G) and genotype distributions (2G/2G, 2G/1G and 1G/1G) were compared between patients and controls using the StatCalc program. Results reveal that the 2G allele is the major allele in Thai population. No statistical association between alleles or genotypes and disease occurrence was seen, both with and without gender stratification. In conclusion, a SNP in the promoter of MMP1 was not associated with knee OA susceptibility in a Thai population. |
