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Sexing and genetic diversity in the genus Charadrius based on CHD gene |
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| รหัสดีโอไอ | |
| Creator | Supattra Poeaim |
| Title | Sexing and genetic diversity in the genus Charadrius based on CHD gene |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 6 |
| Journal No. | 2 |
| Page no. | 141 |
| Keyword | sex identification, chromo-helicase-DNA binding (CHD) gene, Charadrius spp. |
| ISSN | 8578664 |
| Abstract | Charadrius spp. are migratory shorebirds in Thailand during the non-breeding season in which adult plumages are not clearly sexually dimorphic. In this work, a molecular approach was used to determine the gender and genetic diversity. Amplification of intron length polymorphisms in chromo-helicase-DNA-binding (CHD) genes on both Z and W chromosomes of three Charadrius spp. included 87 lesser sand plover (Charadrius mongolus), 27 greater sand plover (C. leschenaultii) and 3 kentish plover (C. alexandrinus). The resulting PCR products from 2550F/2718R primer showed fragments on a conventional agarose gel electrophoresis with size differences ranging from 200 bp between the two respective ZW allele. Males were identified by the presence of a single band about 650 bp (CHD-Z). Heterogametic females were identified by the presence of a second additional fragment length of approximately 450 bp (CHD-W). The CHD-Z fragments were purified and analysed by sequencing. The multiple sequence comparison was accomplished using ClustalX and PHYLIP software. These data demonstrated that the CHD-Z sequence correlated with morphology and can be used for genetic diversity study, of which the results are important for shorebird conservation. |
