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การยับยั้งการสร้างไบโอฟิล์มจากแอคติโนมัยสีทน้ำเค็ม (Anti-Biofilm from Marine Actinomycetes) |
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| รหัสดีโอไอ | |
| Creator | กันตินันท์ ลีธนศักดิ์สกุล |
| Title | การยับยั้งการสร้างไบโอฟิล์มจากแอคติโนมัยสีทน้ำเค็ม (Anti-Biofilm from Marine Actinomycetes) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 422 |
| Keyword | Actinomycetes, anti-biofilm, marine |
| ISSN | 8578664 |
| Abstract | Biofilm is a complex architecture of bacterial group on solid surface. Biofilm is widely important problem in many areas such as medical, marine animal farm and marine transportation. Marine actinomycetes are well-known as good resources for discovery of new secondary metabolites which are useful in pharmaceutical industry. The aim of the present study was to isolate and identify marine actinomycetes having antibiofilm activity. One hundred and one marine actinomycetes were isolated from marine sediments collected at the Gulf of Thailand. From morphological study and partial 16S rRNA sequence analysis, 85 isolates belong to the genus Streptomyces and 16 isolates are rare actinomycetes including Actinopolymorpha, Dietzia, Mycobacterium, Micromonospora and Nocardiopsis. All strains were tested for anti-biofilm activity in 96-well microtiter plate against biofilm producers i.e. Escherichia coli, Pseudomonas aeruginosa, Staphycoccus aureus and Vibrio harveyi. Thirteen strains of marine actinomycetes could inhibit biofilm formation of S. aureus (12.87%), 11 strains inhibited E. coli (10.89%) and 11 strains against V. harveyi (10.89%). However, none of the isolates could reduce biofilm formation of P. aeruginosa. When proteolytic activity was determined, Nocardiopsis sp. GKU 215, Streptomyces sp. GKU 223, Streptomyces sp. GKU 258, Streptomyces sp. GKU 264 and Streptomyces sp. GKU 297 could produce protease. The results may suggest that protease could involve in anti-biofilm production against those pathogenic bacteria. |
