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Genetic manipulation of microalgae for improvement of biodiesel production |
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| รหัสดีโอไอ | |
| Creator | Supachai Vuttipongchaikij |
| Title | Genetic manipulation of microalgae for improvement of biodiesel production |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2555 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 5 |
| Journal No. | 2 |
| Page no. | 130 |
| Keyword | microalgae, biodiesel, genetic transformation, forward genetics, reverse genetics |
| ISSN | 8578664 |
| Abstract | Microalgae are considered the most promising source for biodiesel production for the near future. Various characteristics that promote their potential include high and rapid biomass production rate, ability to accumulate high amount of lipid up to 80% of their cell mass, low cultivation cost and no competition for arable land for crop plants. Selection of high performance strains is the important key for efficient biodiesel production with competitive production cost. However, strains isolated from nature so far only possess one or a few of the required characteristics. Therefore, they are insufficient for the performance demands for biodiesel production. To obtain microalgal strains that are comprised of the required key characteristics, genetic manipulation is the most plausible approach to reach this goal. In this review, key characteristics of microalgae that are required for efficient biodiesel production are discussed for their possibility to be manipulated by either mutagenesis or gene transformation. Forward and reverse genetics in microalgal system in functional genomic era are described regarding the benefits obtained from genome sequences that will contribute to gene function analysis and algal strain improvement. Furthermore, each genetic transformation method is described regarding its pros and cons. Finally, the bottlenecks in many aspects of microalgal strain improvement are underlined as future challenges. |
