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The molecular marker of exposure in the horn snail (Cerithidea cingulata) for determining 17[Beta]-estradiol (E2) contamination in aquatic environments |
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| รหัสดีโอไอ | |
| Creator | Chuta Boonphakdee |
| Title | The molecular marker of exposure in the horn snail (Cerithidea cingulata) for determining 17[Beta]-estradiol (E2) contamination in aquatic environments |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2555 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 5 |
| Journal No. | 2 |
| Page no. | 159-165 |
| Keyword | horn snail, biomarker, Cerithidea cingulata, 17?-estradiol, E2 |
| ISSN | 8578664 |
| Abstract | To search for a molecular marker of exposure for the detection of estrogenic-endocrine disrupting chemicals (e-EDCs) contaminated in aquatic environments, cDNA-AFLP method was performed in horn snail (Cerithidea cingulata). DNA banding differences between the mature horn snails exposed to 100 ng/l 17?-estradiol (E2) and the control group (no E2) were determined. A cDNA-AFLP fragment designated "217_AFLP" appeared only in the E2-treated animals was then selected. The deduced amino acid sequences of the "217_AFLP" aligned against the GenBank database showed high similarity (46% identities) to a transposase gene of the Marbled crab (Pachygrapsus marmorats). Sem-quantitative RT-PCR was analyzed using the relative expression ratio between the "217_AFLP" and the 28S rRNA from foot tissue of mature horn snails. The results showed that the relative expression levels of mature females and males exposed to E2 at 10 ng/l for 7 days were 1.45?0.06 and 1.27?0.18, respectively, which were significantly higher than that of the control group (0.45?0.01 and 0.61?0.06, respectively, P<0.05). Therefore, the "217_AFLP" in mature horn snails can be used as a sensitive biomarker of exposure for detecting E2 contamination in aquatic environments. |
