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Sex identification of some parrots |
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| รหัสดีโอไอ | |
| Creator | Supattra Poeaim |
| Title | Sex identification of some parrots |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2555 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 5 |
| Journal No. | 2 |
| Page no. | 194 |
| Keyword | sex identification, chromo-helicase-DNA binding (CHD) gene, parrot |
| ISSN | 8578664 |
| Abstract | Sex identification of sexually mono morphic avian species for captive breeding by morphological traits alone has a high risk of misidentification. Therefore, sex identification using molecular techniques is recommended, using intron length polymorphism of chromo-helicase-DNA binding (CHD) gene located on the Z (CHD-Z) and W (CHD-W) homologous chromosomes. In this study, using FTA? card, genomic DNA was collected from blood samples of sun conure (Aratinga solstitialis), monk parakeet (Myiopsitta monachus) and crimson-bellied conure (Pyrrhura perlata). The 2550F/2718R primer pair was used to amplify the intronic region of the CHD gene and the resulting PCR products were analyzed by agarose gel electrophoresis. Samples from the females produced two bands, which are 500 bp (CHD-W) and 650-700 bp (CHD-Z), whereas samples from the males produced only a single band at around 650-700 bp (CHD-Z). Thus, this technique can be used for sex identification of parrots, which is required for successful captive breeding programms, as they do not exhibit sexual dimorphism. |
